On-Campus and Other UC Campuses $20/sample. LBNL and CHORI (+10%) $22/sample. Off-Campus (+59%) $31.80/sample *Bioinformatics included in price
Our full plasmid sequencing method has had over 95% success rates for plasmids less than 15~20kb. Please note that large plasmids (20-50kb) requires special handling to minimize sharing and nicking.
Please email your plasmid sequences in FASTA format for reference mapping if available.
Noon on 2nd Thursday of each month
Circular DNA only. No linear DNA
The purified plasmid DNA should be in nuclease free water or 10mM Tris-HCl pH 7.5-pH8.0
· Submit 20ul of 50ng/ul plasmids in 1.5 ml Eppendorf tubes or a 96well plate
· Please label the top of the tube with a permanent marker as clearly as possible with alpha-numeric characters only
· Bring your samples to 310 Barker Hall or ship via FedEx
· Fill out and email Full Plasmid Sequencing order form or Full Plasmid Sequencing-Plate order form to firstname.lastname@example.org. · · · Include a copy with your samples
Samples will be put in queue till we have enough samples to fill two 96well plates
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