GENOMIC DNA PURIFICATION

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Custom Genomic DNA Prep: 96well block format only.  

$300/block for UC labs. 

We sell the culture blocks at our Facility, along with gas permeable membrane covers for $4.50. 

We only accept "Costar 3961" culture blocks, as they are compatible with our instrument. Please ensure you use the correct blocks!

This service utilizes magnetic bead technology for high-throughput purification of genomic DNA. 

We cannot accept culture blocks on the weekend, but if you have access you can leave them in the cold room on the 3rd Floor, ALONG WITH YOUR PAPERWORK... and please email us to let us know they are there. 

To take advantage of this, you will need:

1. 96-well 2.2 ml deep well culture block with gas permeable seal (pick these up in 310 Barker)
2. Up to 95 samples (please reserve well A1 for our sequencing control).
3. Cultures grown in 1~1.5mL of 2YT broth or Terrific broth with antibiotic in deep well culture block. 
4. Start from either a single colony or a glycerol stock, and culture with shaking at 37°C to mid or late log phase (16-20 hours).
5. Please bring your culture block with your downloaded  Plasmid Prep Order Form to 310 Barker Hall.
6. Once the plasmid prep is complete, we can perform the sequencing reaction for you.  Please indicate clearly which primer(s) you need us to use, and provide sufficient amount of 10μM concentration primer (unless it is one of our stock primers).
7. Please email a copy of  the dowloaded Full Plate Sequencing Form to dnaseq@berkeley.edu