FOR PCR REACTIONS CONSIST OF A SINGLE PRODUCT OVER 100BP:
We use magnetic SPRI bead technology to purify PCR products >100 bp, and elute in 40μl H2O (enough for 6-7 sequencing reactions)
Submit PCR products in 10μl total volume according to these guidelines:
Please ensure that all of your samples are normalized to the same volume. If you need more than 10 μl cleaned up, indicate this on your order form.
By Plate: $66/plate
1. Up to 95 samples (we need well A1 for sequencing control).
2. Please indicate on your order form the volumes of your PCR reactions.
3. If you want to sequence your clean PCR products, please provide primers at 10 μM concentration and indicate on your order form where each primer should be placed. If you have more than 3 or 4 primers per plate, it is best to provide us with a corresponding primer plate (aliquot 5ul of 1μM primer per well).
4. To submit your PCR reactions, you will need to use a 96 well half skirted or semi skirted PCR plate.
6. Please download the PCR Cleanup Order Form, submit the form with your samples and e-mail the form to firstname.lastname@example.org.
By Tube: $2.00/sample, minimum charge $20 per order for UC labs
1. Please indicate on your order from the volume of your PCR reactions.
2. If you want us to sequence the samples after cleanup, please provide your sequencing primers at 1 or 10 μM concentration and indicate on your order form where each primer should be placed.
3. Please download and fill out the PCR Cleanup by Tube Order Form and submit the form with your samples.
FOR PCR REACTIONS CONSIST OF A SINGLE PRODUCT< 100BP:
Please contact Hitomi directly
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