Nanopore sequencing
We do daily Nanopore Sequencing. If you have a library of samples, or even just a single plasmid you need fully sequenced, we do Rapid Barcoding runs every day. You can expect a couple thousand reads from this service. If you need MORE READS, then each week we perform a Native Barcoding once a week, where customers receive about 10x the number of reads (typically about 20,000 per sample). If you n
We utilize Oxford Nanopore MinION devices to provide our customers with rapid whole plasmid sequencing data every day. Whether you need one thousand or one million reads, we can help you screen or sequence your DNA targets. In the future we will be offering direct RNA sequencing -- so stay tuned!
Don't struggle with figuring out all of the nuance associated with the often fickle flow cells. Let u
Open-access Instruments
This is our open-access TapeStation 4200 from Agilent Technologies. It is an open-access instrument. Once trained, customers book time on the instrument, and supply all required reagents and consumables. We also provide a dedicated plate vortexter and microfuge to mix and spin down samples prior to analysis. Briefly, the Agilent 4200 TapeStation automates DNA and RNA quality control, delivering fa
The QX200 Droplet Reader is able to obtain absolute quantification for copy number in a variety of PCR based assays. End users supply all of their own reagents and supplies, and we provide the equipment and the required reader oil. Once trained you can reserve time on the shared calendar, and use at your leisure. For more information on the device you can visit Bio-Rad.
A longtime standard for PCR quantification, qPCR is a gold-standard technology for many diagnostic procedures. In theory you can detect single molecules! Like the TapeStation 4200 and the Bio-Rad QX200 droplet digital PCR equipment, the CFX96 is open-access. Once trained, end users supply all requisite consumables and reagents, and the Facility provides access to the equipment. Two units are contr
Some Facility Instruments
OUR WORKHORSE FOR SANGER SEQUENCING: The Applied Biosystems 3730xl is a high-throughput, capillary-electrophoresis analyzer for Sanger DNA sequencing and fluorescent DNA fragment analysis. It can process large sample sets in 96-capillary formats, supporting applications such as microsatellite/STR analysis, SNP genotyping, mutation detection, AFLP, and human-identification testing. Its automated pl
The BioTek Synergy HTX is a multimode microplate reader for absorbance, fluorescence, and luminescence measurements in plates from 6 to 384 wells. It’s used for assays such as nucleic-acid and protein quantification, ELISAs, enzyme kinetics, and cell-growth or toxicity studies. Incubation and shaking support kinetic and cell-based assays; optional reagent injectors enable inject-and-read workflows
ONE OF OUR THREE ROBOTS: The Beckman Coulter Biomek FXP is an automated liquid-handling workstation designed for high-throughput laboratory workflows. Its dual-head configuration combines a 96- or 384-channel pipetting head for rapid plate transfers with a Span-8 head for flexible individual-well pipetting. It can automate tasks such as reagent dispensing, sample transfers, and assay or genomics s
data
Promega's GenePrint 10 fragment analysis kit generates an STR profile by measuring the size of fluorescent PCR fragments at multiple human loci. The resulting electropherogram displays peaks representing detected alleles; comparing the pattern with a reference profile helps verify cell-line identity and identify possible cross-contamination. Everyone should validate their cell lines before, during
An ABI 3730 electropherogram displays fluorescent signals from DNA fragments as they pass through the capillaries and are separated by size. In Sanger sequencing, the colored peaks represent the DNA bases in order, allowing the sequence to be read and assessed for quality; in fragment analysis, peaks show the sizes and relative amounts of labeled DNA fragments.