NANOPORE SEQUENCING

WHAT WE SEQUENCE:

PLASMIDS, LARGE AMPLICONS, AND BACMIDS. WE ACCEPT COLONY PICKS

sample submission deadlines

2PM EVERY TUES & THURS FOR PURIFIED PLASMIDS, AMPLICONS, AND BACMIDS

10AM EVERY TUES & THURS FOR COLONY PICKS AND SAMPLES THAT REQUIRE RCA

DNA qunatification guideline:

PLEASE USE QUBIT ASSAY or PROMEGA QuantiFluor dsDNA SYSTEM TO MEASURE YOUR DNA CONCENTRATION. NANODROP MEASUREMENT IS NOT ACCURATE ENOUGH FOR NANOPORE SEQUENCING. PLEASE SEE THE FILE "CORRELATION NANODROP QUBIT" BY CLICKING DOWNLOAD LINK BELOW.

IF YOU DON'T HAVE QUBIT ASSAY OR QuantFluor SYSTEM, PLEASE REQUEST IT.

service rates:

On-Campus and Other UC Campuses $15/sample.

LBNL and CHORI (+10%) $16.50/sample.

Off-Campus (+60.5%) $24.08/sample

Price includes pre-treatment of plasmids, library prep and Nanopore run, and Bioinformatics

SAMPLE SUBMISSION GUIDELINES:

All samples should be in water or TE:

How much to submit:

Colony picks ~10ul

Purified plasmids 20ul at 50ng/ul

Amplicons 20ul at 50ng/ul or less

Bacmids 20ul at 100ng/ul or more

Please email your plasmid sequences in FASTA format for reference mapping if available.

Sample names:

Letters,Numbers, and " _ " only

No long names

HOW TO PLACE ORDERS:

· Submit 20ul of 50ng/ul plasmids and amplicons in 1.5 ml Eppendorf tubes or a 96well plate

· Please label the top of the tube with a permanent marker as clearly as possible with alpha-numeric characters only

· Bring your samples to 310 Barker Hall or ship via FedEx

· Fill out and email Full Plasmid Sequencing order form or Full Plasmid Sequencing-Plate order form to dnaseq@berkeley.edu. · · · Include a copy with your samples

TURNAROUND TIme:

Run starts every Tuesdays and Thursdays at 2PM

The results ready next afternoon

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