PLASMIDS, LARGE AMPLICONS, AND BACMIDS. WE ACCEPT COLONY PICKS
2PM EVERY TUES & THURS FOR PURIFIED PLASMIDS, AMPLICONS, AND BACMIDS
10AM EVERY TUES & THURS FOR COLONY PICKS AND SAMPLES THAT REQUIRE RCA
PLEASE USE QUBIT ASSAY or PROMEGA QuantiFluor dsDNA SYSTEM TO MEASURE YOUR DNA CONCENTRATION. NANODROP MEASUREMENT IS NOT ACCURATE ENOUGH FOR NANOPORE SEQUENCING. PLEASE SEE THE FILE "CORRELATION NANODROP QUBIT" BY CLICKING DOWNLOAD LINK BELOW.
IF YOU DON'T HAVE QUBIT ASSAY OR QuantFluor SYSTEM, PLEASE REQUEST IT.
On-Campus and Other UC Campuses $15/sample.
LBNL and CHORI (+10%) $16.50/sample.
Off-Campus (+60.5%) $24.08/sample
Price includes pre-treatment of plasmids, library prep and Nanopore run, and Bioinformatics
All samples should be in water or TE:
How much to submit:
Colony picks ~10ul
Purified plasmids 20ul at 50ng/ul
Amplicons 20ul at 50ng/ul or less
Bacmids 20ul at 100ng/ul or more
Please email your plasmid sequences in FASTA format for reference mapping if available.
Letters,Numbers, and " _ " only
No long names
· Submit 20ul of 50ng/ul plasmids and amplicons in 1.5 ml Eppendorf tubes or a 96well plate
· Please label the top of the tube with a permanent marker as clearly as possible with alpha-numeric characters only
· Bring your samples to 310 Barker Hall or ship via FedEx
· Fill out and email Full Plasmid Sequencing order form or Full Plasmid Sequencing-Plate order form to firstname.lastname@example.org. · · · Include a copy with your samples
Run starts every Tuesdays and Thursdays at 2PM
The results ready next afternoon
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