UC Berkeley DNA Sequencing Facility

510.642.6383

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    • SEQUENCING
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  • More
    • HOME
    • SERVICES
      • SEQUENCING
      • FREE STOCK PRIMERS
      • GENOTYPING
      • PLASMID & GENOMIC DNA
      • PCR REACTION CLEANUP
      • CELL LINE AUTHENTICATION
      • DNA QUANTIFICATION
      • DNA NORMALIZATION
      • NANOPORE SEQUENCING
      • NGS LIBRARY PREP
      • hPSC GENETIC ANALYSIS
    • CUSTOM ASSAYS & SERVICES
      • GOT WAGYU?
      • CUSTOM SERVICE REQUEST
    • REAGENTS + Pols FOR SALE
    • ORDER FORMS
    • PRICING
    • Plates&blocks
    • OPEN ACCESS INSRUMENTS
    • DNA TEAM
UC Berkeley DNA Sequencing Facility

510.642.6383

  • HOME
  • SERVICES
    • SEQUENCING
    • FREE STOCK PRIMERS
    • GENOTYPING
    • PLASMID & GENOMIC DNA
    • PCR REACTION CLEANUP
    • CELL LINE AUTHENTICATION
    • DNA QUANTIFICATION
    • DNA NORMALIZATION
    • NANOPORE SEQUENCING
    • NGS LIBRARY PREP
    • hPSC GENETIC ANALYSIS
  • CUSTOM ASSAYS & SERVICES
    • GOT WAGYU?
    • CUSTOM SERVICE REQUEST
  • REAGENTS + Pols FOR SALE
  • ORDER FORMS
  • PRICING
  • Plates&blocks
  • OPEN ACCESS INSRUMENTS
  • DNA TEAM

PLASMID & Genomic DNA PURIFICATION

High Copy Plasmid Prep: 96well block format only.  $120/block for UC labs


We accept only "Costar 3961" Culture block that is compatible with our instrument.


This service utilizes magnetic bead technology for high-throughput purification of plasmid DNA from E. coli. It works well for high copy plasmids.  For low copy plasmids, please see the special instruction below for growing the cultures.


We cannot accept culture blocks on the weekend.


To take advantage of this, you will need:

  1. 96-well 2.2 ml deep well culture block with gas permeable seal (pick these up in 310 Barker)
  2. Up to 95 samples (please reserve well A1 for our sequencing control).
  3. Cultures grown in 1~1.5mL of 2YT broth or Terrific broth with antibiotic in deep well culture block. 
  4. Start from either a single colony or a glycerol stock, and culture with shaking at 37 oC to mid or late log phase (16-20 hours).
  5. Please bring your culture block with your downloaded  Plasmid Prep Order Form to 310 Barker Hall.
  6. Once the plasmid prep is complete, we can perform the sequencing reaction for you.  Please indicate clearly which primer(s) you need us to use, and provide sufficient amount of 10μM concentration primer (unless it is one of our stock primers).
  7. Please email a copy of  dowloaded Full Plate Sequencing Form to dnaseq@berkeley.edu


For Low Copy Plasmid Prep, please grow your culture in culture tubes using Terrific broth or 2YT broth + antibiotic (NO LB broth) with shaking  for 16-20 hours and transfer 2ml of the culture in a 96well block.


Custom Genomic DNA Prep: 96well block format only.  $150/block for UC labs. 


Please contact Juan Carlos directly to inquire


media recipe

2X YT Media Recipe (1000 ml)

1M Potassium Phosphate Solution (1000ml)

2X YT Media Recipe (1000 ml)

1. Mix:

  • 16g Bacto Tryptone
  • 10g Bacto Yeast Extract
  • 5g NaCl
  • 900ml dH2O

2. Adjust pH to 7.0 with 5N NaOH

3. Bring the volume to 1000mo with dH2O 

4. Sterilize by autoclaving.


Terrific Broth (1000ml)

1M Potassium Phosphate Solution (1000ml)

2X YT Media Recipe (1000 ml)

1. Mix following four ingredients and sterilize by autoclaving

  • 12 g Bacto-tryptone, 
  • 24 g Bacto-yeast extract, and 
  • 4.0 ml glycerol
  • 900ml dH2O

2. Allow liquid to cool to less than 60oC

3. Add 100ml of 1M Potassium phosphate solution




1M Potassium Phosphate Solution (1000ml)

1M Potassium Phosphate Solution (1000ml)

1M Potassium Phosphate Solution (1000ml)

1. Mix:

  • 0.17 Molar monobasic potassium phospate (23.135 grams/liter KH2PO4)
  • 0.72 Molar dibasic potassium phosphate (125.410 grams/liter K2HPO4).

2. Sterile filter this solution through a 0.22 micron filter OR autoclave

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