We perform human Cell Line Authentication / Identification for you using short tandem repeat DNA profiling (STR Profiling).
This assay will NOT WORK for mouse or any other mammalian cell line.
Give yourself the peace of mind that your cells are what you think they are and have been identified and characterized -- before publishing your results. Validate your cells or cell lines before spending time, energy, and money on experiments.
$100 per cell line for UC labs. Add 10% for LBNL and CHORI. Add 61% for others.
The service includes: Genomic DNA extraction, QC, PCR amplification of 9 STR loci plus Amelogenin using Promega GenePrint 10 System, Fragment Analysis with ABI 3730XL DNA Analyzer, comprehensive data analysis with ABI GeneMapper software and final verification using supplier databases including ATCC and DSMZ.
We start the CLA process every Thursday. Turnaround time: 3~5 business days.
You may send samples via any overnight courier (or even economy 2-day if there's enough dry ice included). HOWEVER, if you are shipping Wednesday for Thursday delivery (we begin processing samples Thursday mornings) we recommend FedEx because packages are typically delivered by 10:30am -- enabling delivered samples to be tested the same day. Samples that arrive Thursday afternoon (or later) will be processed the following week -- unless there are more than 20 samples samples in your shipment.
Absolutely NO submission of known or potentially infectious biologicals: no cells with any known or suspected or possible viruses or other potentially hazardous agents. All samples will be handled in a BSL1 laboratory. If ATCC (or anyone else) notes the cells to be BSL2 (or higher), we can still perform the CLA analysis for you. HOWEVER, you would need to submit cell lysates to eliminate any possibility of infectious agent transmission to our staff or within our facility. Thank you for your understanding and adherence to this important policy.s
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GUIDELINES FOR SUBMITTING CELL LINES FOR FRAGMENT OR SHORT TANDEM REPEAT (STR) ANALYSIS:
Thank you very much for following these basic guidelines -- It really helps!
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