GENOTYPING
Before starting your project, please ensure that your dye sets are compatible with our Applied Biosystems 3730XL DNA Analyzers.
The dye set best suited for our 3730XL is DS-33, which includes these dyes: 6-FAM, VIC, NED, PET, and LIZ (LIZ Size Standard). Please call us if you must use other dye sets.
To submit your samples, you will need to use a 96 well half skirted or semi skirted PCR plate.
We prefer samples to be submitted in a specific type of plate:
- Catalog#: 4ti-0770
- Description: FrameStar 96, semi-skirted
- You can order from Thomas Scientific on Bearbuy
- If you are on campus, please stop by 310 Barker and we are happy to provide the plates for free of charge.
Please provide a minimum of 10 μl of PCR products in HiDi Formamide. Each well should have size standard.
Applied Biosystems has Peak ScannerTM software v1.0 freely available to download https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=catNavigate2&catID=603624
Please download the Geotyping Order Form and submit the form with your samples. Also please e-mail the form to dnaseq@berkeley.edu.
Sample names cannot have spaces and only include the following punctuation: -+.(){}_
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