UC Berkeley DNA Sequencing Facility

510.642.6383

  • HOME
  • SERVICES
    • SEQUENCING
    • FREE STOCK PRIMERS
    • GENOTYPING
    • PLASMID & GENOMIC DNA
    • PCR REACTION CLEANUP
    • CELL LINE AUTHENTICATION
    • DNA QUANTIFICATION
    • DNA Normalization
    • FULL PLASMID SEQUENCING
  • REAGENTS FOR SALE
  • FORMS
  • DNA TEAM
  • PRICING
  • Plates&blocks
  • OPEN ACCESS INSRUMENTS
  • More
    • HOME
    • SERVICES
      • SEQUENCING
      • FREE STOCK PRIMERS
      • GENOTYPING
      • PLASMID & GENOMIC DNA
      • PCR REACTION CLEANUP
      • CELL LINE AUTHENTICATION
      • DNA QUANTIFICATION
      • DNA Normalization
      • FULL PLASMID SEQUENCING
    • REAGENTS FOR SALE
    • FORMS
    • DNA TEAM
    • PRICING
    • Plates&blocks
    • OPEN ACCESS INSRUMENTS
UC Berkeley DNA Sequencing Facility

510.642.6383

  • HOME
  • SERVICES
  • REAGENTS FOR SALE
  • FORMS
  • DNA TEAM
  • PRICING
  • Plates&blocks
  • OPEN ACCESS INSRUMENTS

GENOTYPING

Before starting your project, please ensure that your dye sets are compatible with our Applied Biosystems 3730XL DNA Analyzers.


The dye set best suited for our 3730XL is DS-33, which includes these dyes: 6-FAM, VIC, NED, PET, and LIZ (LIZ Size Standard). Please call us if you must use other dye sets.


To submit your samples, you will need to use a 96 well half skirted or semi skirted PCR plate.  


We prefer samples to be submitted in a specific type of plate:

  • Catalog#: 4ti-0770/c
  • Description: FrameStar 96, semi-skirted
  • To order, please contact Dennis Harte @ 510-882-0640 or www.BasicScientific.com
  • If you are on campus, please stop by 310 Barker and we are happy to provide the plates for free of charge.

Please provide a minimum of 10 μl of PCR products in HiDi Formamide. Each well should have size standard.


Applied Biosystems has Peak ScannerTM software v1.0 freely available to download  https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=catNavigate2&catID=603624


Please download the Geotyping Order Form and submit the form with your samples. Also please e-mail the form to dnaseq@berkeley.edu.


Sample names cannot have spaces and only include the following punctuation:  -+.(){}_

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